{"created":"2023-07-27T04:41:22.570455+00:00","id":23701,"links":{},"metadata":{"_buckets":{"deposit":"a7a4d481-1238-43ab-a95d-5970b128dfe5"},"_deposit":{"created_by":3,"id":"23701","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"23701"},"status":"published"},"_oai":{"id":"oai:tohoku.repo.nii.ac.jp:00023701","sets":["89:178"]},"author_link":["57823","57827","57868","57826","57828","57824","56282","57825"],"item_4_alternative_title_20":{"attribute_name":"その他の言語のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Expression of heat shock proteins induced by cyclical stretching stress in human periodontal ligament fibroblasts"}]},"item_4_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2000-12","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"115","bibliographicPageStart":"108","bibliographicVolumeNumber":"19","bibliographic_titles":[{"bibliographic_title":"東北大学歯学雑誌"}]}]},"item_4_date_62":{"attribute_name":"登録日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2008-05-02","subitem_date_issued_type":"Created"}]},"item_4_date_63":{"attribute_name":"公開日（投稿完了日）","attribute_value_mlt":[{"subitem_date_issued_datetime":"2008-05-02","subitem_date_issued_type":"Created"}]},"item_4_date_65":{"attribute_name":"発行日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2000-12","subitem_date_issued_type":"Created"}]},"item_4_date_80":{"attribute_name":"更新日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2010-01-27","subitem_date_issued_type":"Created"}]},"item_4_description_15":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_4_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"本研究は, in vitroにてヒト歯根膜線維芽細胞(Periodontal ligament fibroblast : PDLF)に周期的伸展力を負荷した場合の種々のストレスタンパク質(Heat shock protein : HSP)の発現を調べることにより, メカニカルストレスによる歯周組織の改造機構におけるストレスタンパク質の発現誘導を明らかにすることを目的とした。実験には健全な小臼歯から採取した4種類のPDLFを用いた。細胞をシリコーンメンブレン底面を持つ培養ディッシュ上で培養し, 1秒間stretch, 1秒間relax, 15%elongationの周期で伸展力を72時間負荷した。ストレスタンパク質の発現は, 電気泳動後, 抗HSP47,60,70モノクロール抗体を用いてウェスタンプロッティング法にて調べた。その結果, ストレスタンパク質の発現パターンは2種類認められ, ALPase活性が低い値を示したPDLF-1,2は周期的伸展力負荷によりHSP47,60,70の発現を有意に増強したのに対して, ALPase活性が高い値を示したPDLF-3,4は周期的伸展力負荷によってもその発現をほとんど変化させなかった。これらの結果より, PDLFのストレスタンパク質の発現はメカニカルストレスにより制御されているが, その発現パターンは個々のPDLFの機能発現の相違により異なることが示唆された。","subitem_description_type":"Abstract"},{"subitem_description":"The purposes of this study were to examine the induction of heat shock proteins(HSPs)47,60,and 70 in human periodontal ligament fibroblasts(PDLFS)subjected to cyclical stretching stress in vitro and to elucidate the expression of HSPs in periodontal tissue subjected to mechanical stress.PDLFS obtained from healthy human premolars, freshly extracted from 4 orthodontic patients, were examined.The PDLFS were incubated inα-MEM medium containing 10% fetal bovine serum and were seeded in 6-well flexible silicon bottom plates coated with type I collagen.Cyclical stretching stress (1 sec stretching/1 sec relaxation, 15% elongation) was applied to the PDLFS for 72 hours.Cell homogenate was subjected to lO% SDS polyaclylamide gel electrophoresis, and HSP production was analyzed by Western blotting methods, using anti-HSP47,60,or 70 antibody.Two types of HSP production patterns were observed in response to cyclical stretching stress.PDLFS that expressed low alkaline phosphatase(ALPase)activity showed marked increases in HSP47,60,and 70 production, while PDLFS that expressed high ALPase activity showed no significant change in HSP production.These results indicate that the expression of HSPs is regulated by mechanical stress whereas the expression profile of HSPs differs among the individual types of PDLFS.","subitem_description_type":"Abstract"}]},"item_4_description_41":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"紀要類(bulletin)","subitem_description_type":"Other"}]},"item_4_description_66":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"738733 bytes","subitem_description_type":"Other"}]},"item_4_full_name_2":{"attribute_name":"著者(ヨミ)","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"57826","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Okazaki, Masako"}]},{"nameIdentifiers":[{"nameIdentifier":"57827","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Shimizu, Yoshiyuki"}]},{"nameIdentifiers":[{"nameIdentifier":"57828","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Chiba, Mirei"}]},{"nameIdentifiers":[{"nameIdentifier":"56282","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Mitani, Hideo"}]}]},"item_4_publisher_34":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"東北大学歯学会"}]},"item_4_radio_69":{"attribute_name":"公開範囲","attribute_value_mlt":[{"subitem_radio_item":"学外"}]},"item_4_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"02873915","subitem_source_identifier_type":"ISSN"}]},"item_4_source_id_9":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00081101","subitem_source_identifier_type":"NCID"}]},"item_4_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"岡崎, 雅子"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"清水, 義之"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"千葉, 美麗"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"三谷, 英夫"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-02-10"}],"displaytype":"detail","filename":"KJ00000093805.pdf","filesize":[{"value":"738.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KJ00000093805.pdf","url":"https://tohoku.repo.nii.ac.jp/record/23701/files/KJ00000093805.pdf"},"version_id":"385f7348-71dc-43e0-a1ee-80cecf3354c5"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"heat shock protein","subitem_subject_scheme":"Other"},{"subitem_subject":"cyclical stretching","subitem_subject_scheme":"Other"},{"subitem_subject":"periodontal ligament fibroblasts","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"周期的伸展力によるヒト歯根膜線維芽細胞のストレスタンパク質の発現に関する研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"周期的伸展力によるヒト歯根膜線維芽細胞のストレスタンパク質の発現に関する研究"}]},"item_type_id":"4","owner":"3","path":["178"],"pubdate":{"attribute_name":"公開日","attribute_value":"2008-05-02"},"publish_date":"2008-05-02","publish_status":"0","recid":"23701","relation_version_is_last":true,"title":["周期的伸展力によるヒト歯根膜線維芽細胞のストレスタンパク質の発現に関する研究"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-07-27T19:33:50.900889+00:00"}