{"created":"2023-07-27T04:41:22.825935+00:00","id":23707,"links":{},"metadata":{"_buckets":{"deposit":"35143900-6e67-438e-9e7b-6e761ca9cfb3"},"_deposit":{"created_by":3,"id":"23707","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"23707"},"status":"published"},"_oai":{"id":"oai:tohoku.repo.nii.ac.jp:00023707","sets":["89:178"]},"author_link":["57852","57851"],"item_4_alternative_title_20":{"attribute_name":"その他の言語のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Regulation of CD80 expression on human monocytes via apoptosis mediated by superantigen and lipopolysaccharide"}]},"item_4_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2000-12","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"174","bibliographicPageStart":"165","bibliographicVolumeNumber":"19","bibliographic_titles":[{"bibliographic_title":"東北大学歯学雑誌"}]}]},"item_4_date_62":{"attribute_name":"登録日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2008-05-02","subitem_date_issued_type":"Created"}]},"item_4_date_63":{"attribute_name":"公開日（投稿完了日）","attribute_value_mlt":[{"subitem_date_issued_datetime":"2008-05-02","subitem_date_issued_type":"Created"}]},"item_4_date_65":{"attribute_name":"発行日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2000-12","subitem_date_issued_type":"Created"}]},"item_4_date_80":{"attribute_name":"更新日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2010-01-27","subitem_date_issued_type":"Created"}]},"item_4_description_15":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_4_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"スーパー抗原であるstaphylococcal enterotoxin B(SEB)は単球のアポトーシスを誘導し, 内毒素リポ多糖(LPS)はこれを抑制する。SEB刺激で誘導される単球のアポトーシスは抗CD95(Fas)抗体, 抗CD95リガンド(CD95L)抗体により抑制されることから, SEBはFasの経路を介したアポトーシスを誘導することが示唆された。また, SEB刺激によるCD80^+単球の増加と関連して, CD80^-単球の方がCD80^+単球よりもアポトーシスに高い感受性を示し, CD80^+単球が選択的に増加することが示された。一方, SEB刺激で丁細胞からインターフェロン-γ(IFN-γ)が産生される。IFN-γは単独でCD80を単球に誘導する。IFN-γの作用は, 抗CD119(IFN-γレセプターα鎖)抗体を用いたIFN-γレセプターのブロックによるCD80^+単球の減少により確かめられた。また, LPSはインターロイキン(IL)-10の作用を介してCD80の発現を抑制した。しかしながら, SEB刺激で誘導されるCD80^+単球はIFN-γが産生される前から緩やかに増加すること, 抗FasL抗体処理によるアポトーシス抑制によってCD80^+単球が減少することから, このCD80^+単球の増加は, SEB刺激に対する単球の複合的な反応の結果であることが示唆された。すなわち, SEB処理により, IFN-γが産生される以前にすでに単球にアポトーシスのシグナルが伝達され, その感受性の違いからCD80^+単球の選択的な増加が起こる。遅れて産生されるIFN-γの作用により, さらに二次的な増加が誘導される。一方, LPSはアポトーシスおよびIFN-γの作用の両方に抑制的に作用し, SEB刺激で誘導されるCD80^+単球の増加を抑制することが示唆された。","subitem_description_type":"Abstract"},{"subitem_description":"Staphylococcal enterotoxin B (SEB) rapidly increased the number of apoptotic monocytes, whereas lipopolysaccharide (LPS) -treated monocytes were resistant to the apoptotic action of SEB. This SEB-induced killing was abolished by anti-CD95 (Fas) and anti-CD95 ligand (FasL) monoclonal antibodies (mAb), suggesting a CD95-based pathway of apoptosis. Apoptosis in cultures with SEB was affected by differences in the susceptibility of CD80^+ and CD80^- monocytes to apoptosis. On the other hand, interferon-y(IFN-γ) production from activated T cells was observed after 9 hr of stimulation with SEB. Addition of IFN-γ to unstimulated cultures induced a marked increase in the number of CD80^+ monocytes, which was inhibited by LPS through the action of interleukin-10. The numbers of SEB-induced CD80^+ monocytes were partially decreased by anti-CD119 (IFN-y receptor a-chain) mAb and by anti-CD95L mAb an anti-apoptotic agent. These findings show that SEB-induced monocyte apoptosis is closely associated with the enrichment of CD80^+ monocytes generated before IFN-γ production, followed by up-regulation of CD80^+ by IFN-γ, and that LPS has negative effects in both cases. These results may help to elucidate the effects and functions of SEB and LPS in the immune system.","subitem_description_type":"Abstract"}]},"item_4_description_41":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"紀要類(bulletin)","subitem_description_type":"Other"}]},"item_4_description_66":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"804282 bytes","subitem_description_type":"Other"}]},"item_4_full_name_2":{"attribute_name":"著者(ヨミ)","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"57852","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Takahashi, Masahiro"}]}]},"item_4_publisher_34":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"東北大学歯学会"}]},"item_4_radio_69":{"attribute_name":"公開範囲","attribute_value_mlt":[{"subitem_radio_item":"学外"}]},"item_4_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"02873915","subitem_source_identifier_type":"ISSN"}]},"item_4_source_id_9":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00081101","subitem_source_identifier_type":"NCID"}]},"item_4_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"高橋, 昌宏"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-02-10"}],"displaytype":"detail","filename":"KJ00000093811.pdf","filesize":[{"value":"804.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KJ00000093811.pdf","url":"https://tohoku.repo.nii.ac.jp/record/23707/files/KJ00000093811.pdf"},"version_id":"4e382a6b-4789-4ff0-8f92-30617d46565f"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"SEB","subitem_subject_scheme":"Other"},{"subitem_subject":"LPS","subitem_subject_scheme":"Other"},{"subitem_subject":"monocyte apoptsis","subitem_subject_scheme":"Other"},{"subitem_subject":"CD80","subitem_subject_scheme":"Other"},{"subitem_subject":"interferon-γ","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"スーパー抗原ならびに細菌内毒素によるアポトーシスを介した単球のCD80発現調節作用","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"スーパー抗原ならびに細菌内毒素によるアポトーシスを介した単球のCD80発現調節作用"}]},"item_type_id":"4","owner":"3","path":["178"],"pubdate":{"attribute_name":"公開日","attribute_value":"2008-05-02"},"publish_date":"2008-05-02","publish_status":"0","recid":"23707","relation_version_is_last":true,"title":["スーパー抗原ならびに細菌内毒素によるアポトーシスを介した単球のCD80発現調節作用"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-07-27T18:58:36.943247+00:00"}