@article{oai:tohoku.repo.nii.ac.jp:00065519,
 author = {LI, Qi and KIJIMA, Akihiro},
 issue = {1/2},
 journal = {Tohoku journal of agricultural research},
 month = {Nov},
 note = {application/pdf, The induction of gynogenetic tetraploids by the suppression of the meiotic division in the Pacific oyster Crassostrea gigas was attempted in three trials using the treatments of cytochalasin B (0.5μg/mL; CB). The effects of various durations of CB treatment on tetraploid induction frequency were examined. Haploid gynogenesis was induced by sperm which were UV-irradiated for 60s at an intensity of 72erg/mm^2/s. CB treatment was effective in inhibiting meiosis, and the optimum time for the suppression of both the 1st and 2nd polar bodies was from 10 to 45min postinsemination, yielding 51.4% gynogenetic tetraploids. The results of this study suggest the possibility of producing gynogenetic tetraploid Pacific oysters., 紀要類, 685154 bytes},
 pages = {1--10},
 title = {Gynogenetic Tetraploid Larvae of the Pacific Oyster Crassostrea gigas Induced by Inhibition of the 1st and 2nd Meiotic Divisions},
 volume = {57},
 year = {2006}
}