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Absorption of Horseradish Peroxidase (HRP) In Vitro Across Bovine Jejunal and Ileal Epithelia Around the Time of Weaning
http://hdl.handle.net/10097/30055
http://hdl.handle.net/10097/30055ea15eb8d-1c96-4f45-9948-3410abae144c
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
KJ00004368833.pdf (645.8 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||||||||||||||
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| 公開日 | 2008-05-02 | |||||||||||||||||
| タイトル | ||||||||||||||||||
| タイトル | Absorption of Horseradish Peroxidase (HRP) In Vitro Across Bovine Jejunal and Ileal Epithelia Around the Time of Weaning | |||||||||||||||||
| 言語 | ||||||||||||||||||
| 言語 | eng | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||
| 主題 | horseradish peroxidase | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||
| 主題 | absorption | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||
| 主題 | transport | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||
| 主題 | calf | |||||||||||||||||
| 資源タイプ | ||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||
| 資源タイプ | departmental bulletin paper | |||||||||||||||||
| 著者 |
NITTA, Hayato
× NITTA, Hayato
× SUGAWARA, Takuya
× SUGAWARA, Eri
× KOBAYASHI, Yosuke
× KATOH, Kazuo
× OBARA, Yoshiaki
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| 抄録 | ||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||
| 内容記述 | Using the everted sac methodology as well as an Ussing chamber, we investigated changes in the absorption of horseradish peroxidase (EC 1.11.1.7, HRP (40 kDa)) in jejunum and ileum segments isolated from male Holstein cattle around the time of weaning (6 to 15 wks old). By the everted sac method, HRP transport (HRP concentration on the serosal side sampled after a 60-min-incubation) at 15 wks of age was significantly greater than that at 6 wks of age, in both segments of the intestine. Absorption was not significantly different between the jejunum and ileum. Addition of Na^+/K^+ ATPase inhibitor (ouabain, 1 mM) did not cause any significant change in HRP absorption, whereas Na^+/H^+ anti-transporter inhibitor (amiloride, 1μM) significantly increased the absorption in both sacs at 8 wks of age. By the Ussing chamber method, there were no significant differences between the values for Jsm and Jms, while the Jnet value was nearly zero for both epithelia. In addition, the flux (Jms) of Lucifer yellow, a cell-membrane-impermeable fluorescence dye, was significantly greater at 6 than at 13 wks of age in the ileal epithelia, although the flux was significantly greater in the jejunal than the ileal epithelia at both ages. From these findings, we conclude that: 1) bovine jejunal and ileal epithelia are able to absorb a large molecule such as the HRP protein; 2) HRP transport occurs in a concentration-dependent manner and may in part be via a paracellular pathway; 3) the increased HRP transport shown at 15 wks of age may not be caused by an increased use of the paracellular pathway. | |||||||||||||||||
| 書誌情報 |
Tohoku journal of agricultural research 巻 56, 号 1/2, p. 1-10, 発行日 2005-11-25 |
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| ISSN | ||||||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||||||
| 収録物識別子 | 00408719 | |||||||||||||||||
| 書誌レコードID | ||||||||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||||||||
| 収録物識別子 | AA0086391X | |||||||||||||||||
| フォーマット | ||||||||||||||||||
| 内容記述タイプ | Other | |||||||||||||||||
| 内容記述 | application/pdf | |||||||||||||||||
| 著者版フラグ | ||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||
| 出版者 | ||||||||||||||||||
| 出版者 | Faculty of Agriculture, Tohoku University | |||||||||||||||||
| 資源タイプ | ||||||||||||||||||
| 内容記述タイプ | Other | |||||||||||||||||
| 内容記述 | 紀要類(bulletin) | |||||||||||||||||
| 登録日 | ||||||||||||||||||
| 日付 | 2008-05-02 | |||||||||||||||||
| 日付タイプ | Created | |||||||||||||||||
| 公開日（投稿完了日） | ||||||||||||||||||
| 日付 | 2008-05-02 | |||||||||||||||||
| 日付タイプ | Created | |||||||||||||||||
| 発行日 | ||||||||||||||||||
| 日付 | 2005-11-25 | |||||||||||||||||
| 日付タイプ | Created | |||||||||||||||||
| フォーマット | ||||||||||||||||||
| 内容記述タイプ | Other | |||||||||||||||||
| 内容記述 | 645758 bytes | |||||||||||||||||
| 更新日 | ||||||||||||||||||
| 日付 | 2010-01-27 | |||||||||||||||||
| 日付タイプ | Created | |||||||||||||||||
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Cite as
NITTA, Hayato, SUGAWARA, Takuya, SUGAWARA, Eri, KOBAYASHI, Yosuke, KATOH, Kazuo, OBARA, Yoshiaki, 2005, Absorption of Horseradish Peroxidase (HRP) In Vitro Across Bovine Jejunal and Ileal Epithelia Around the Time of Weaning: Faculty of Agriculture, Tohoku University, 1–10 p.
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